By M. Caliskan
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Additional info for Analysis of Genetic Variation in Animals
Cravador, J. Azevedo, and H. Guedes-Pinto. 2001. Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed" Churra da Terra Quente". BIOTECHNOLOGIE AGRONOMIE SOCIETE ET ENVIRONNEMENT 5: 7-16. ; V. Cubric-Curik, K. Schwend, R. Achmann, and J. Slkner. 2006. Genetic characterisation and breed assignment in Austrian sheep breeds using microsatellite marker information. Journal of Animal Breeding and Genetics 123: 265-271. Beckmann, J. ; and J. L. Weber.
2009). The combined PCR products were detected by capillary electrophoresis using an ABI Prism 310 DNA Genetic Analyzer (AppliedBiosystems) and the size of alleles was determined using GeneScan-500 LIZ Size Standard (AppliedBiosystems). A total of 112 different alleles were identified for 10 microsatellites in all seven breeds analyzed (Table 7). The most polymorphic marker was SO005 with a total of 21 alleles, while microsatellite SO101 was the least polymorphic, showing only 7 alleles (Manea et al, 2009).
Journal of animal science 86: 2496. Weber, J. L. 1990. Human DNA polymorphisms based on length variations in simplesequence tandem repeats. Genome analysis 1: 159-181. Weir, B. ; and C. C. Cockerham. 1984. Estimating F-statistics for the analysis of population structure. Evolution: 1358-1370. Wright, S. 1931. Evolution in Mendelian populations. Genetics 16: 97. ; Y. Fu, T. Shi, and J. Wang. 2009. Six Local Sheep Breeds AFLP Analysis of Genetic Diversity in Xinjiang. China Animal Husbandry Veterinary Medicine.
Analysis of Genetic Variation in Animals by M. Caliskan