By Pier Carlo Braga, Davide Ricci
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Extra info for Atomic Force Microscopy: Biomedical Methods and Applications
66, 1–4. 7. Villarrubia, J. S. (1996) Scanned probe microscope tip characterization without cantilever tip characterizers. J. Vac. Sci. Technol. B. 14, 1518–1521. 8. , Czajkowsky, D. , and Shao, Z. (1999) AFM tips: How sharp are they? J. Microsc. 196, 1–5. Artifacts in AFM 37 9. Taatjes, D. , Quinn, A. , Lewis, M. , and Bovill, E. G. (1999) Quality assessment of atomic force microscopy probes by scanning electron microscopy: Correlation of tip structure with rendered images. Microsc. Res. Tech.
Effect of creep in the vertical direction: overshooting at the edges of the step. Fig. 11. The free end of the scanner will follow an arc during scanning, creating a bowl-like image. This effect is especially evident on large scans of flat surfaces. 34 Ricci and Braga and y piezoelectric elements: if they are not both accurately calibrated the image will be affected by a geometrical distortion. It is useful to add that quite often (in fact, always) the sample will have a plane tilt relative to the motion of the scanner.
Has two distinct advantages. The effect of lateral forces is effectively eliminated (important because cells have low resistance to shear stress). Because the interaction now arises from an impulse action at one extreme of the oscillatory motion of the tip, the inertia of the sample will resist deformation. , the free-running frequency) and the width of the resonance envelope. The latter is severely degraded in water, and soft levers are preferred for analysis of live cells. Although resonance mode imaging has many advantages, the jury is probably still out in the case of in vitro analysis of cells (9–11).
Atomic Force Microscopy: Biomedical Methods and Applications by Pier Carlo Braga, Davide Ricci