By Glen Baker, Susan Dunn, Andrew Holt, Abel Lajtha
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Additional info for Handbook of Neurochemistry and Molecular Neurobiology 3rd Edition: Practical Neurochemistry Methods
The most commonly used atypicals include clozapine, olanzapine, risperidone, and quetiapine. Recently, three papers have reported the determination of risperidone and its active metabolite 9‐hydroxyrisperidone using LLE and SPE technologies. The analytical columns used to separate these compounds were C4 or C18 bonded phases of 3 mm or 5 mm particle sizes with UV/VIS detection. Mobile phases consisted of phosphate buffers (pH 3–4) in acetonitrile. The sample volumes used ranged from 200 ml to 1 ml, with extraction recoveries averaging 90%.
J. Odontiadis), the Canada Research Chair Program (Dr. J. Odontiadis), and the Canadian Institutes for Health Research (CIHR) (Ms. G. Rauw) for their financial support. References Aboul‐Enein HY, Al‐Duraibi IA. 1998. Separation of several free polyamines and their acetylated derivatives by ion‐pair reversed‐phase high performance liquid chromatography. Biomed Chromatogr 12: 291-293. Abu Ruz S, Millership J, Heaney L, McElnay J. 2003. Simple liquid chromatography method for the rapid simultaneous determination of prednisolone and cortisol in plasma and urine using hydrophilic lipophilic balanced solid phase extraction cartridges.
A transient diastereomeric complex is formed between the enantiomer and the chiral component in the mobile phase, similar to the complexes formed with chiral stationary phases. A review by Liu and Liu (2002) cites several papers where addition of CPMAs has been used in analyzing amphetamine‐related compounds. Some CPMAs include amino acid enantiomers, metal ions, proteins, and cyclodextrins. Advantages of this method of analysis include the use of less expensive columns and more flexibility in the optimization of chiral separation (Misˇl’anova´ and Hutta, 2003).
Handbook of Neurochemistry and Molecular Neurobiology 3rd Edition: Practical Neurochemistry Methods by Glen Baker, Susan Dunn, Andrew Holt, Abel Lajtha