Introduction to Confocal Fluorescence Microscopy, Second by Michiel Mueller PDF

By Michiel Mueller

ISBN-10: 0819460435

ISBN-13: 9780819460431

This booklet presents a complete account of the idea of picture formation in a confocal fluorescence microscope in addition to a realistic guide to the operation of the software, its obstacles, and the translation of confocal microscopy facts. The appendices supply a short connection with optical conception, microscopy-related formulation and definitions, and Fourier theory.

Contents

- Symbols and abbreviations
- Preface
- Confocal fluorescence microscopy
- Implementation
- functional limits
- Digitization
- Miscellaneous topics
- Appendix A: parts of optical theory
- Appendix B: formulation, kinfolk and definitions
- Appendix C: Fourier theory
- Bibliography
- Index

Show description

Read Online or Download Introduction to Confocal Fluorescence Microscopy, Second Edition PDF

Similar instruments & measurement books

Get Shaping the Day: A History of Timekeeping in England and PDF

Timekeeping is a vital task within the glossy global, and we take it with no consideration that our lives our formed via the hours of the day. but what turns out so traditional this present day is admittedly the intense final result of centuries of technical innovation and movement of principles approximately time. Shaping the Day is a pathbreaking learn of the perform of timekeeping in England and Wales among 1300 and 1800.

Materials Characterization: Introduction to Microscopic and - download pdf or read online

This publication covers state of the art thoughts normal in smooth fabrics characterization. very important points of characterization, fabrics constructions and chemical research, are integrated. regular strategies, reminiscent of metallography (light microscopy), X-ray diffraction, transmission and scanning electron microscopy, are defined.

Read e-book online The Direction of Time PDF

Ever a resource of philosophical conjecture and debate, the idea that of time represents the thrashing middle of physics. This ultimate paintings via the celebrated physicist Hans Reichenbach represents the fruits and integration of a lifetime's philosophical contributions and inquiries into the research of time.

Get Characterization of high Tc materials and devices by PDF

It is a transparent and up to date account of the applying of electron-based microscopies to the examine of high-Tc superconductors. Written via major specialists, this compilation presents a accomplished overview of scanning electron microscopy, transmission electron microscopy and scanning transmission electron microscopy, including information of every method and its purposes.

Additional info for Introduction to Confocal Fluorescence Microscopy, Second Edition

Sample text

Perpendicular to the optical axis, that coincides with the geometrical focal point. The right panel shows an axial plane centered on the optical axis. Logarithmic contrast stretching equivalent to that in Fig. 7 has been applied to enhance the visibility of any side lobe structures. detection pinhole suppresses virtually the entire lateral side lobe structure, whereas some axial contributions remain. Image formation in a confocal microscope follows the same principle as for the wide-field microscope.

When multiple labeled specimens are used. The degree to which chromatic aberrations influence the imaging properties strongly depends on the microscope objective and the range of wavelengths used in excitation and detection. Microscope objectives are generally corrected for either two (achromats) or three (apochromats) wavelengths (see Sec. 1). This means that for these particular wavelengths, the aberrations are optimally balanced and minimized. Between these wavelengths, the microscope objective generally still performs well, but outside this wavelength region the aberrations usually grow exponentially, particularly towards the shorter wavelengths.

2, the speed of image acquisition in a confocal fluorescence microscope can be increased considerably by using slit excitation and detection. However, this compromises the axial resolution. Wilson (1995) calculated the axial response to an infinitely thin fluorescing layer as a function of the width, vw , of the detection slit (Fig. 9). 4. 6 when compared to pinhole detection with a pinhole diameter equal to the slit width. 4 Fluorophores Confocal fluorescence microscopy relies on the use of fluorophores to provide image contrast.

Download PDF sample

Introduction to Confocal Fluorescence Microscopy, Second Edition by Michiel Mueller


by Christopher
4.4

Rated 4.48 of 5 – based on 40 votes